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Laboratory

The chart above shows the curve Crop growth of bacteria at 37 º C. Hourly samples were taken from the crop (black dots), and diluted in water so that all of them containing a final concentration of 10,000 organisms per milliliter. The samples were divided into three tubes of 1 ml each and treated as follows:

Tube 1: Nothing added. Control tube.
Tube 2: addition of chloramphenicol, an inhibitor of translation
Barrel 3: addition of penicillin

After leaving acting antibiotic for twenty minutes at 37 ° C, aliquots of 100 microliters of each tube and inoculated in Petri dishes with fresh medium and without antibiotic. After 24 hours at 37 ° C were counted and colony forming units on plates. The table below shows the results.

Note: The explanation is not due to the emergence of mutants resistant to antibiotics or any other type of genetic phenomenon

Explains

results

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Nature vs Biodiesel

A research team has isolated a microorganism of a pond whose average temperature is 25 º C. When studying the location of the microorganism in the water column of the lake with respect to day-night cycles, researchers found the following result (solid line on graph).

In the laboratory the research group working with the organism isolated and determined the following data on their physiology and metabolism. It is a microaerophilic bacterium strict heterotrophic metabolism, whose optimum temperature is 25 º C. This research group attempts to reproduce in the laboratory the results observed in nature simulating cycles day and night in a column of water inoculated with this organism, which is placed in a chamber incubated aerobically at 25 ° C temperature and in which by simulated solar light day and night cycles but you get a completely different result (dashed line on graph): Microorganisms are always at the same depth.


a. - Develop a hypothesis to explain the result.
b. - Design an experiment in which you can check this hypothesis

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inoculated with a population of 1,000 bacteria in a culture medium. The conditions are so ideal and perfect that microorganisms do not go through a conditioning phase or latency and begin to grow and divide from the outset.

At different times they are taken samples for analysis, measuring the optical density of culture (OJ, broken line), and the number of cells present in the crop (N, solid line). The values \u200b\u200bwere fed into a computer that is in a semilog plot.

Unfortunately, unconfigure a computer virus data table and produced two sets of graphs are different.

Which of the two graphs depicted above is correct and why?