The chart above shows the curve Crop growth of bacteria at 37 º C. Hourly samples were taken from the crop (black dots), and diluted in water so that all of them containing a final concentration of 10,000 organisms per milliliter. The samples were divided into three tubes of 1 ml each and treated as follows:
Tube 1: Nothing added. Control tube.
Tube 2: addition of chloramphenicol, an inhibitor of translation
Barrel 3: addition of penicillin
After leaving acting antibiotic for twenty minutes at 37 ° C, aliquots of 100 microliters of each tube and inoculated in Petri dishes with fresh medium and without antibiotic. After 24 hours at 37 ° C were counted and colony forming units on plates. The table below shows the results.
Tube 2: addition of chloramphenicol, an inhibitor of translation
Barrel 3: addition of penicillin
After leaving acting antibiotic for twenty minutes at 37 ° C, aliquots of 100 microliters of each tube and inoculated in Petri dishes with fresh medium and without antibiotic. After 24 hours at 37 ° C were counted and colony forming units on plates. The table below shows the results.
Note: The explanation is not due to the emergence of mutants resistant to antibiotics or any other type of genetic phenomenon
results
Explains
0 comments:
Post a Comment